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We are interested in how metabolism influences placental development and function in both normal and complicated pregnancies.

Our Research

Overview

Our lab is based in the Department of Obstetrics & Gynaecology at the University of Cambridge. We are interested in all things placenta! You can find out more about recent projects below. Please contact us if you're interested in collaborating or if you'd like to discuss studentship or job opportunities.

Research

Metabolic control of epigenetics regulating trophoblast development

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Cell metabolism is conventionally viewed as a means of obtaining bioenergy for cellular homeostasis or building blocks for biomass growth. However, metabolic intermediates can also strongly influence gene expression through additional non-bioenergetic functions, for instance as rate-limiting substrates or co-factors in a variety of epigenetic processes.

Our goal is to understand the mechanistic links between metabolism and epigenetic programs directing trophoblast stemness and differentiation. How and which metabolites moonlight as epigenetic substrates and what are the environmental factors which regulate their metabolism?

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Metabolite sensing and response

Metabolites have additional functions as cell signaling molecules which provides a means of communicating the cellular status among different organelles and allows for metabolic pathways to be integrated to cellular function. 

Our work aims to understand how trophoblasts sense and respond to changes in nutrient and metabolite availability, and delineate the pathways.

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Placental sex differences and pregnancy outcome

Pregnancies carrying male or female fetus may differ in their risk of miscarriage, preterm birth, preeclampsia and fetal growth restriction. As a fetal organ, placental sex reflects that of the fetus. The placental transcriptome differs considerably based on fetal sex.

Our previous work has shown that sex differences in the placental transcriptome are largely driven by genes that escape X chromosome inactivation (XCI). One XCI escapee regulates polyamine metabolism and protects female trophoblasts from cellular stress. Understanding these sex differences may hold the key to understanding fetal sex differences in the susceptibility and severity of pregnancy disorders. 

Read more about placental sex differences.

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Understanding metabolism to improve placental health

Our long-term goal is to establish deep mechanistic insights into how placental metabolism regulates placental development. By leveraging on our understanding  of placental metabolism we hope to design therapeutic strategies based around nutrition to promote placental growth and optimal function and reduce the risk of pregnancy complications. 

Team

Principal Investigator

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Team

Group Leader / MRC Career Development Fellow -

Department of Obstetrics & Gynaecology

PI - Centre for Trophoblast Research

Affiliate PI - Cambridge Stem Cell Institute

Graduate Students

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Giulia Avellino, MSc

PhD Candidate - Funded by the CTR

Research Assistants

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Stephanie Rogers, MSc

Methodologies

Human trophoblast stem cells and primary trophoblasts

3D Trophoblast Organoids

Animal models of pregnancy and placenta-specific gene targeting

Profiling post-translational modification

Chromatin Profiling

Metabolomics

Methodologies

Single cell sequencing

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Publications

The human placenta exhibits a unique transcriptomic void

Sungsam Gong, Francesca Gaccioli, Irving LMH Aye, Giulia Avellino, Emma Cook, Andrew RJ Lawson, Luke HR Harvey, Gordon CS Smith, D Stephen Charnock-Jones

Cell Reports 2023 Jul 25;42(7):112800

Physiologically relevant culture medium Plasmax improves human placental trophoblast stem cell function

Giulia Avellino, Ruhi Deshmukh, Stephanie N Rogers, D Stephen Charnock-Jones, Gordon CS Smith, Saverio Tardito, 

Irving LMH Aye

American Journal of Physiology: Cell Physiology 2023 Apr 1;324(4):C878-C885

Placental sex-dependent spermine synthesis regulates trophoblast gene expression through acetyl-coA metabolism and histone acetylation

Irving LMH Aye, Sungsam Gong, Giulia Avellino, Roberta Barbagallo, Francesca Gaccioli, Benjamin J Jenkins, Albert Koulman, Andrew J Murray, D Stephen Charnock-Jones, Gordon CS Smith

Communications Biology 2022 Jun 15;5(1):586

Placental energy metabolism in health and disease-significance of development and implications for preeclampsia

Irving LMH Aye, Catherine E Aiken, D Stephen Charnock-Jones, Gordon CS Smith

American Journal Obstetric Gynecology 2022 Feb 226(2S):S928-S944

Insulin increases adipose adiponectin in pregnancy by inhibiting ubiquitination and degradation: impact of obesity

Irving LMH Aye, Fredrick J Rosario, Anita Kramer, Oddrun Kristiansen, Trond M Michelsen, Theresa L Powell, Thomas Jansson

Journal of Clinical Endocrinology and Metabolism 2022 Jan 1;107(1):53-66

Adiponectin supplementation in pregnant mice prevents the adverse effects of maternal obesity on placental function and fetal growth

Irving LMH Aye, Fredrick J Rosario, Theresa L Powell, Thomas Jansson

Proneedings from the National Academies of Science USA 2015 Oct 13;112(41):12858-63

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